Fig. 4. TcBR-C isoform-specific effects. (A) Day 1 seventh-instar larvae were injected with indicated isoform-specific dsRNAs and subjected to RT-PCR as prepupae. Knocking down one isoform had no effect on the others. (B-D) Effects of knocking down TcBR-C isoforms. Black arrowheads mark the position to which wings normally extend in wild-type pupae (see Fig. 2A). Depletion of either Z1 (B) or Z2 (C) individually causes shortening of the wings (black arrows) and legs (white arrows), with the Z2 effect being stronger. Also note the aberrant head position (white arrowhead) and the blistered wing (asterisk). Combined double RNAi (D) enhanced the effect of isoform-specific dsRNAs on wings, legs and gin traps (insets), such that the aberrant pupae display the mild phenotypes of common-region TcBR-C RNAi (see Fig. 2I). Scale bars: in B, 1 mm for B-D; in insets, 200 µm.