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Figure 5


Fig. 5. Loss of TcMet induces upregulation of TcBR-C mRNA in precocious prepupae. RT-PCR shows that low TcBR-C expression in feeding day 1 eighth-instar larvae (L8) rises at the prepupal stage (PP8) 48 hours later (see also Fig. 1C). Injection of TcMet dsRNA to early-fifth instar larvae induced premature sixth-instar prepupae (PP6), which contained reduced TcMet mRNA and strongly elevated TcBR-C expression. Controls injected with egfp dsRNA and analyzed on day 1 of the seventh instar (L7) displayed a low TcBR-C mRNA level, typical for feeding larvae.