Fig. 8. CpBR-C RNAi prevents proper differentiation of pupal characters. Chrysopa larvae were injected with CpBR-C dsRNA at the end of the first instar and, after they arrested at the prepupal stage, the larval cuticle was removed. Examples of their heterochronic phenotypes are compared with the normal larval, pupal and adult situation. (A-D) Tarsi (tar) of CpBR-C(RNAi) individuals (D) have distinct borders between segments, as in pupae and adults, whereas their distal tip (pretarsus) retains the larval character and is narrow, with a long arolium (ar). Claws (cl) are large and hooked as in larvae or adults. (E-H) Dorsal abdominal cuticle with short thorns resembles that of the larva. The thorns in CpBR-C(RNAi) (H) are densely packed because the animal had been compressed in its old larval cuticle. (I-L') Instead of larval stemmata (st), CpBR-C(RNAi) animals develop compound eyes, also present in pupae and adults. Compared with pupa, eye differentiation is more advanced, as indicated by the formation, although incomplete, of ommatidial lenses (arrows, compare J' with L'). Also note the perforations (arrowheads) on the CpBR-C(RNAi) eye (L). Scale bars: 500 µm (J); 200 µm (B,C,K); 100 µm (A,D,I,L); 50 µm (E-H,J',L'); 20 µm (K').