Fig. 1. E-cadherin becomes ectopically expressed, whereas vimentin is diminished in palate and nasal mesenchyme in Zeb1 mutant mice. (A) Zeb1 immunostaining is shown in mesenchyme in the palate (P) and developing nasal cartilage (N). (B) E-cadherin immunostaining is present on the nasal (N), palatal (P) and tongue (T) epithelium. (C) Vimentin immunostaining is shown in palatal and nasal mesenchyme. (D) E-cadherin immunostaining is seen ectopically on nasal and palatal mesenchyme in Zeb1-null mice. Note the failure of palate closure. The arrow indicates mesenchyme in the nasal region. (E) Loss of vimentin immunostaining in the palatal and nasal mesenchyme of Zeb1-null mice. Sections of mice at E16.5 are shown. Scale bars: 100 µm.