Fig. 3. Ectopic E-cadherin expression and diminished vimentin and GFAP expression in the embryonic eye of Zeb1-null mice. (A) Immunostaining for Zeb1. Retina (R); forming eye muscles (M); lens (L); cornea (C); eye lid (EL). (B) E-cadherin immunostaining is confined to the epithelium of the lens and cornea. (C) Vimentin immunostaining in the retina, lens, optic nerve (ON) and forming eyes muscles. (D) GFAP immunostaining in the retina, optic nerve and forming eye muscles. (E) Ectopic immunostaining for E-cadherin in the retina, optic nerve and forming eye muscle in Zeb1-null mice. (F) Double immunostaining for vimentin (red) and GFAP (green) shows loss of expression of both proteins in the retina, optic nerve and forming eye muscle in Zeb1-null mice. Sections of mice at E16.5 are shown. Scale bars: 100 µm.