Fig. 4. Mutation of Zeb1 leads to ectopic expression of E-cadherin, loss of vimentin and GFAP expression, and decreased proliferation in the ventricular zone of the brain. (A) Immunostaining for Zeb1 in the ventricular zone (arrow) of the lateral ventricle. (B,C) Immunostaining for vimentin and GFAP, respectively, in the ventricular zone. (D) Immunostaining for both vimentin (red) and GFAP (green) is lost in the ventricular zone of Zeb1 mutant mice. (E) Immunostaining for E-cadherin is not evident in the lateral ventricle in wild-type mice. (F,G) Ectopic immunostaining for E-cadherin in the ventricular zone of the lateral ventricle in Zeb1-null mice. (H) Immunostaining for E-cadherin in the third ventricle in Zeb1-null mice. (I) BrdU immunostaining in the ventricular zone of the lateral ventricle. (J,K) Double immunolabeling of the boxed region in I for BrdU and Zeb1, respectively. (L) Overlay of J and K. (M) Quantitation of BrdU incorporation into the ventricular zone of the left lateral ventricle (LLV), right lateral ventricle (RLV), hypothalamus (Hypo.) and, as a control, the tongue. For a representative view of areas counted, see Fig. S3 in the supplementary material. Sections at E15.5 are shown. Scale bars: 100 µm in E,F,I; 50 µm in A-D,G,H; 25 µm in J-L.