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Figure 5


Fig. 5. Disruption of PI3K and PAK pathway activation in PDGFR{alpha}PI3K/PI3K cells. (A-C) Western blot analysis of whole cell lysates from primary mesenchymal cells. (A) PDGFR{alpha} is expressed and ERK1/2 is phosphorylated in PDGFAA and serum-stimulated control and PDGFR{alpha}PI3K/PI3K cells. (B) Akt and S6K1 are not phosphorylated in PDGFAA-stimulated PDGFR{alpha}PI3K/PI3K cells. (C) PAK1 is not phosphorylated in PDGFAA stimulated PDGFR{alpha}PI3K/PI3K cells. Loading controls (immediately beneath the sample) were either actin (for the PDGFR{alpha}) or stripped blots that were reblotted for the unphosphorylated form of the protein. Stimulation conditions: st, starved; AA, 10 ng/ml PDGF-AA; se, 10% serum. Blots are representative of three independent experiments.