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Figure 2


Fig. 2. Both PSE1 and PSE2 contribute to PRE activity. (A) Construct P[en2] contains 8 kb of upstream en sequences with PSE1 and PSE2 flanked by LoxP (L) and FRT (F) sites, respectively. (B-I) Immunoperoxidase staining detecting β-gal protein in embryos from a P[en2] line and its derivatives obtained by treating with Flp and/or Cre recombinase. Embryos shown are 6 hours AEL (B-E) and 10 hours AEL (F-I); anterior is leftwards, dorsal is upwards. All are lateral views, except C, which is a ventrolateral view. Expression is initiated correctly in all lines, then β-gal is misexpressed in cells between the stripes in {Delta}PSE2, {Delta}PSE1 and {Delta}both lines. Deletion of PSE2 causes relatively minor misexpression (G). Deletion of PSE1 is more extensive (H), and deletion of both causes the most misexpression between the stripes (I).