Fig. 6. Effects of RA signaling and Ad4BP/SF-1 on the expression ER and CYP26A1. (A-K) Beads soaked in RA (B,G), RAA (C,H) and DMSO (D,I), and chick embryonic fibroblasts expressing Ad4BP/SF-1 (E,J) and AP (F,K) were implanted into embryos of both sexes as described in Figs 2 and 3. An untreated embryo is shown in A. The embryos were fixed at stage 27, and the expression of ER (A-F) and CYP26A1 (G-K) was examined by whole-mount in situ hybridization. Sections of female (ZW) samples are shown. Arrowheads in B and C, and in G and H, indicate affected expression of ER and CYP26A1, respectively. Scale bars: 100 µm.