Fig. 2. PLK-1 cytoplasmic asymmetry in early C. elegans embryos. Merged immunofluorescence micrographs of DAPI staining (red) with anti-PLK-1 (A-F) and anti-PLK-2 (M,N) staining (green). (G-L) GFP fluorescence of GFP::PLK-1^PBD. (O,P) GFP fluorescence of GFP::PLK-1^FL (O) and GFP::PLK-2^FL (P). Closed and open arrowheads indicate the germline blastomere and its somatic sister, respectively. Stages of embryos in A-L are marked to the left; M, 1C; N-P, 2C. Scale bar: 10 µm.