Fig. 4. Expression of RFT1 in Hd3a RNAi plants under SD
conditions. (A) Expression of Hd3a in Hd3a RNAi
and wild-type plants. Hd3a expression was suppressed at all
developmental stages in Hd3a RNAi plants. (B) Expression of
RFT1 in Hd3a RNAi and wild-type plants. RFT1
transcription was very low in wild-type plants (black line). However, in
Hd3a RNAi plants, RFT1 expression (green, blue and red
lines) had increased 30 days before flowering, the stage required for
promoting reproductive transition. Leaves were collected from wild-type plants
and Hd3a RNAi plants at 35, 50, 70 and 90 DAS (ZT 0) under SD
conditions.