Fig. 1. Sporulation induced by cytokinins. (A) Developed KP cells were incubated with various concentrations of isopentenyl adenine (triangles), discadenine (filled circles) or zeatin (squares). Cells were treated with PKA inhibitors 10 µM H89 (open circles) or 5 µM myristoylated PKI (crosses) 45 minutes prior to addition of isopentenyl adenine. Spores were counted after 1 hour. (B) Developed KP cells were incubated with various concentrations of the artificial cytokinins, thidiazuron (squares), kinetin (open circles) and 6-benzyl-aminopurine (triangles), and the number of spores determined after 1 hour. Each experiment was repeated three to five times.