Fig. 1. Stage 10B egg chamber maturation in vitro is influenced by Cox1 inhibitors and exogenous PGs. (A) Normal egg chamber at stage 10B, (B) stage 12 or (C) stage 14. (D-K) Extent of development characteristic of stage 10B egg chambers matured in vitro and treated as indicated. (D) Control, develops to stage 14 with only slight reduction in dorsal appendages; (E-I) aspirin blocks maturation in a dose-dependent manner. (J) 150 µM NS-398 blocks in vitro development; (K) partial rescue of developmental block induced by 150 µM NS-398 by PGH₂ at 20 µM. Anterior is towards the left in all images. Asterisks indicate nurse cell region; arrows indicate dorsal appendages. (L) Effects of Cox1 inhibitors and exogenous PGs on egg chamber development in vitro. Scale bar: 50 µM. `Stalled' was defined as incomplete nurse cell breakdown in these experiments. t-tests were performed comparing drug treated (blue bar) to drug plus prostaglandin (yellow, green or red). Flu, fluprostenol.