Fig. 5. pxt mutants exhibit actin-related defects throughout oogenesis. (A) Normal ovariole and (B) shortened pxt ovariole with missing stages, fused chambers (bracket) and degenerating chambers (star). (C) Multi-nucleate nurse cells in pxt egg chambers as a result of membrane breakdown. Nurse cell nuclei are outlined and arrows indicate actin-rich aggregates containing residual ring canals, compare with S8 in A. (D) Loss of follicular separation and germarium defects in older pxt mutant females. Broken line indicates germarium-stage cysts. (E) Defective border cell migration due to lagging cells (outlined, compare with `BC' in A). (F) Nurse cells further away from the oocyte have membrane instability (outlined in yellow) and actin aggregates (arrow), whereas those closer to the oocyte have more normal actin filament formation (outlined in white). (A-F) Projections of confocal sections. Green is Hts (1B1) and Fas3 (7G10); red is phalloidin and blue is DAPI. A, anterior; P, posterior. Scale bars: 50 µM.