Fig. 4. Multipotent differentiation capacities of EG cells established in AKT-MER transgenic mice. (A) Hematoxylin and Eosin (HE) staining of teratomas derived from EG cells established without bFGF. The cells differentiated into various tissues, including squamous epithelia (left), glands (middle) and cartilage (right, arrowhead). (B) HE staining of teratomas derived from EG cells established without bFGF and SCF. The teratomas were composed of various tissues, including cartilage (left), mucosal glands (middle) and muscle (right). Scale bar: 100 µm. (C) In vitro hematopoietic cell differentiation. EG cells established in the absence of bFGF (left), and the absence of bFGF and SCF (right), produced various hematopoietic cells in the OP9 differentiation system. Scale bar: 50 µm. (D) Chimeras derived from the transgenic EG cells established without bFGF. The contribution of the EG cells could be assessed by monitoring the EGFP fluorescence. In the E12.5 embryos (left and middle), EGFP fluorescence was distributed in the entire body. Right, newborn non-chimeric (middle) and chimeric mice (left, right). L, live; D, dead at birth. Left chimera survived to adult. (E) Chimeras derived from transgenic EG cells established without bFGF and SCF. Shown is the E12.5 chimera.