Fig. 3. Expression patterns of genes associated with early development in mouse Rex1^+/- populations. (A) Ratio of expression levels of early genes in Rex1⁺ and Rex1^- populations were examined by Q-PCR. The averages of values from RNA of Rex1⁺ and Rex1^- cells sorted three times independently are shown in the graph. (B) Immunostaining of Rex1-EGFP/Oct3/4-ECFP double-knock-in cells with antibodies for proteins expressing in the peri-implantation embryos. Fluorescence of antibody reaction is shown as red signal (Alexa 594) for Nanog, Klf4 and Tbx3, or purple signal (Alexa 647) for Esrrb and Foxd3.