Fig. 4. Rump associates with nos mRNA in vivo. (A) RT-PCR to detect nos in RNA co-immunoprecipitated from wild-type (WT) and rump¹ Drosophila ovaries with either anti-Rump or anti-β-galactosidase antibody. Poly(A+) RNA serves as positive control for RT-PCR. Reactions were performed with (+) and without (-) reverse transcriptase and products were visualized with ethidium bromide. Molecular weight standards in the first lane correspond to the 1 kb-Plus Ladder (Invitrogen). Beneath is shown an immunoblot of protein from each IP sample with anti-Rump antibody. (B) RT-PCR for nos (top) and anti-Rump immunoblot (bottom) as in A. Samples from WT (W) and rump¹ (r) 0- to 2-hour embryos are indicated. Reactions were carried out with (+) or without (-) reverse transcriptase (RT) using total RNA (T) from the extracts used for IP as a positive control or RNA co-immunoprecipitated with anti-Rump antibody.