Fig. 5. Distribution of Rump protein in Drosophila ovaries and embryos. (A,B) Confocal images of stage-10 egg chambers from wild-type (A) and rump¹ (B) females stained with anti-Rump antibody (red). The actin cytoskeleton is visualized in green, DNA in blue. nc, nurse cells; fc, follicle cells; oo, oocyte. (C,D) Immunohistochemical staining of preblastoderm wild-type (C) and rump¹ (D) embryos with anti-Rump antibody. (E-F') Confocal images showing the posterior poles of blastoderm-stage wild-type (E) and rump¹ (F) embryos stained with anti-Rump (red) and anti-Vas (green). DNA is in blue. (E',F') Red channel (Rump) only from E and F. In wild-type embryos, Rump is detected cytoplasmically and in all somatic nuclei, but it is specifically absent from nuclei of pole cells, which are marked by Vas (arrowheads).