Fig. 3. deps-1 mutants display temperature-sensitive sterility and embryonic lethality. (A) Fertility profiles of deps-1(bn121) M-Z- at 15, 20 and 24.5°C. Mutant hermaphrodites were picked to individual plates as L4s, and the fertility of each animal scored several days later. Animals that generated (a) >20 or (b) 1-20 larval progeny are defined as fertile. Animals that did not have larval progeny but (c) laid dead eggs, (d) produced oocytes or oocyte-like material, or (e) failed to make either embryos or oocytes are defined as sterile. Numbers of adults examined were 45 (15°C), 132 (20°C) and 105 (24.5°C). (B) Number and quality of eggs laid by deps-1(bn121) M+Z- and M-Z- adults at 15 and 20°C and deps-1(bn121) M+Z- adults at 24.5°C. n.d., not determined.