Fig. 4. DEPS-1 promotes the accumulation of glh-1 and rde-4 mRNA and protein. (A-D) Wild-type and deps-1(bn124) germ lines stained with PA3, a marker for chromatin (A,C), and anti-GLH-1 (B,D). A-D are stacks of three confocal sections taken at 1 µm intervals showing germ nuclei present on the surface of the pachytene region of the hermaphrodite germ line. Scale bar: 10 µm. (E) Western blot analysis (top panel) of GLH-1 in whole worm extracts, with -tubulin as a loading control, and histogram (lower panel) of glh-1 mRNA (measured by quantitative RT-PCR) and GLH-1 protein levels in deps-1 mutants compared with wild type. (F) Similar analysis of rde-4 mRNA and RDE-4 protein levels, but using partially purified worm extracts, and comparing with rde-4 mutants and with wild type. The histograms show average ratios obtained in two independent experiments. The wild-type ratio was set at 1.0 in each experiment. For mutants, error bars indicate the s.e.m.