Fig. 1. Embryonic dorsoventral patterning is unaffected by the absence of HSPG synthesis. (A-E) Dark-field images of cuticles from (A) wild-type and (B-E) mutant larvae. (F-H) Dorsal and lateral views of eggs laid by wild-type and ttv mothers. Anterior is to the left. (A) The ventral cuticle of wild-type larvae displays a regular segmental pattern of denticle belts alternating with bands of naked cuticle. (B) Larvae mutant for hh (as well as wg) develop a continuous lawn of denticles due to loss of naked cuticle. (C) sfl germline clone (glc) larvae lacking maternal and zygotic N-deacetyl/N-sulfotransferase activity show loss of naked cuticle and fused denticles resembling the hh mutant phenotype, but no increase in denticle belt width or DV patterning defects. (D) ttv glc larvae display a similar phenotype, with no abnormalities in DV patterning. (E) The phenotype of a ttv mutant larva from a depleted mother that lacks somatic and germline ttv activity resembles ttv glc mutants. (F) Paired paddle-shaped dorsal appendages are located posterior to the operculum (line) at the anterior of the wild-type eggshell. (G) Eggshell morphology is unaffected by loss of ttv activity in the germline. (H) Eggs laid by Ttv-depleted females are shorter and have a smaller operculum (black bar), phenotypes associated with reduced Dpp signaling activity in follicle cells.