Fig. 2. The coelomocytes of sm237 mutant C. elegans contain aberrant endosomes. (Aa-f) The coelomocyte uptake is reduced in the unc-108(sm237) mutant. The uptake of ssGFP in the coelomocyte of a wild-type animal (a,b), unc-108(sm237) mutant (c,d) and unc-108(RNAi) animal (e,f) carrying P_myo-3ssgfp were examined by visualizing the GFP accumulation in the body cavity and coelomocytes. White dashed line indicates the outline of the coelomocyte and arrows point to the body cavity with accumulated GFP. (Ba-Df) sm237 animals contain abnormal endosomes. The coelomocytes of a wild-type animal (a,b), unc-108(sm237) mutant (Bc-Bf, c,d in C,D), unc-108(RNAi) animal (Bg-Bj, e,f in C,D) carrying different endosome and lysosome markers were examined. In wild-type coelomocyte, RME-8::GFP (B) associates with early and late endosomes; LMP-1::GFP (C) mostly stains lysosomes and GFP::RME-1 (D) marks recycling endosomes (arrows). Scale bars: 2.5 µm.