Fig. 3. Fat and Hippo signaling regulate the nuclear localization of Yki. (A-H') High magnification views of imaginal discs, stained for Yki (red), nuclei (DAPI, blue) and containing mutant clones marked by absence of GFP (green). (A) wts^X1, (B) Mer⁴; ex^e1, (C) fat^G-rv, (D) ex^e1, (E) ex^e1 fat^G-rv, (F) hpo^42-47, (G) mats^e235 and (H) fat^G-rv. Arrows indicate clone edges. (I-J') High magnification views of imaginal discs expressing RNAi lines under ci-Gal4 control, stained for Yki (red), nuclei (DAPI, blue) and Engrailed (green). ci-Gal4 drives expression in anterior cells (yellow line), partially complementary to Engrailed. (I) ci-Gal4 UAS-ex RNAi UAS-dcr2. (J) ci-Gal4 UAS-fat RNAi UAS-dcr2.