Fig. 4. A Chc+ transgene suppresses aux eye and wing mutant phenotypes. (A) aux^K47/aux^D128 Drosophila wing. (B) aux^K47/aux^D128 wing with one copy of PChc+. The wing is indistinguishable from that of wild type. (C) Bar chart showing the degree of suppression of aux^K47/aux^D128 eyes by a single copy of PChc+. For each genotype, 51-124 facets in each of ten eyes were examined. Error bars represent one standard deviation from the mean calculated for each eye. The effect of PChc+ is significant (Student's t-test, P<0.001). (D-F) Tangential sections of adult eyes are shown as examples of data tabulated in C. PChc+ complements Chc¹ completely (Bazinet et al., 1993). The mutant phenotype of aux hypomorphs reflects some defects in lateral inhibition (see Fig. 2E), and mainly defects in R-cell restriction. The same phenomenon was observed for lqf hypomorphs (Overstreet et al., 2004). This observation might reflect a particular requirement for efficient Delta signaling in R-cell restriction, or mechanistic differences in signaling at different times during eye development.