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Figure 5


Fig. 5. Subcellular distribution of concentrated Shh::GFP ligand in apical region. (A-I) High magnification of apical region in 8-somite-stage mouse neural tubes of indicated genotypes. Nuclei (blue), Shh::GFP ligand (green), {gamma}-tubulin ({gamma}-tub, red), polaris (turquoise). Images are single confocal slices. Association of Shh::GFP ligand, {gamma}-tubulin and polaris is shown by arrows. Shh::GFP ligand continues to associate with the basal body region in Smo and Skn mutants. (J) Three-dimensional surface rendering of six confocal images taken sequentially along the z-axis. Note the adjacent, non-overlapping co-localization of the three proteins.