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Figure 6


Fig. 6. Association of Shh::GFP ligand with lysosomes and stabilized microtubules. (A-C) High magnification of apical compartment of 8-somite-stage mouse neural tubes of indicated genotype. Nuclei (blue), Shh::GFP ligand (green), Lamp1 (red). The association of Shh::GFP ligand with the lysosome is indicated by the arrows. The bulk of Shh::GFP ligand apical accumulation does not co-segregate with the lysosome. (D-F) Immunostaining for acetylated {alpha}-tubulin reveals a network of stabilized microtubules (red) that span between the apical and basal surfaces of neural progenitors. Shh::GFP (green) is present in the notochord (asterisk). The boxed region in D is shown at high magnification in E,F. Arrows in E indicate a string of Shh::GFP ligand puncta.