Fig. 5. Left-right asymmetry of posterior SHF explants from
Mlc3f-nlacZ-2E embryos at the 6-somite stage. (A-H) Left
(L) (A-D) and right (R) (E-H) explants from the pSHF of
Mlc3f-nlacZ-2E mouse embryos at the 6-somite stage: (A,E) Hoechst
staining; (B,F) myosin heavy chain (MHC) immunofluorescence; (C,G) β-gal
immunofluorescence. (D) Merge of B and C; (H) merge of F and G. (I-P)
Left (I-L) and right (M-P) explants from Mlc3f-nlacZ-9
embryos at the 6-somite stage. (I,M) Hoechst staining; (J,N) MHC
immunofluorescence; (K,O) β-gal immunofluorescence. (L) Merge of J and K;
(P) merge of N and O. Scale bar: 10 µm in P for A-P. (Q) The number
of β-gal and MHC double-positive cells as a percentage of the total
number of cells. White and black columns show the percentage in left and right
pSHF explants, respectively, from Mlc3f-nlacZ-2E (2E) embryos at the
6-somite stage (50 explants from each side) (P
0.03). Grey and
dark-grey columns show the percentage in left and right explants,
respectively, from Mlc3f-nlacZ-9 (9) embryos at the same stage (25
explants for each side). (R) qRT-PCR on ten pooled left (l) or right
(r) explants of pSHF from Mlc3f-nlacZ-2E embryos at the 6-somite
stage after 72 hours of culture. lacZ (arrowheads) and Mlc2a
transcript abundance is expressed as a percentage of that of Gapdh
transcripts.