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Figure 2


Fig. 2. Two different domains of APP exhibit distinct binding properties. AP in situs on E11.5-12.5 brains. Ventral views. Arrowheads indicate olfactory bulbs. Arrows indicate optic tecta. (A) Binding of AP-APP deletion series (numbered according to APP695). Complete AP-APPs{alpha} is AP-APP(18-612). Binding pattern 1 refers to prominently striated staining specifically in the anterior tectum at E9.5. Binding pattern 2 refers to widespread staining of the brain, including throughout the tectum, and prominent olfactory bulb staining, at E11.5-12.5. Colored bars indicate regions of APP with strong binding in patterns 1 (green) and 2 (blue). (B,C) AP-APP(199-345) gave strong tectal staining. AP-APP(199-293) also bound to RGC axons in tecta, though less strongly. (D,E) PI-PLC or mock treatment of brains, then AP-APPs{alpha} staining. PI-PLC treatment eliminated most olfactory bulb staining and some broader staining. (F,G) AP-APP(18-205) and AP-APLP1 exhibit widespread binding including in the tectum, with particular prominence in olfactory bulb. (H) Diagram of chick brain, ventral view.