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Figure 2


Fig. 2. Trunk and tail identities are lost in Smed-bcatenin1-silenced planarians, whereas the dorsoventral axis remains unaffected. (A-F) Analysis of Smed-HoxD (A-D) and Smed-AbdBa (E,F) reveals the disappearance of tail and trunk identities, including the mouth (m). (G,H) Expression of Smed-TCEN49 almost disappears in radial-like hypercephalized planarians. (I-P) Analysis of Septin (I-L) and Eye53 (M-P) expression demonstrates that the dorsoventral axis is not affected. Eye53 is also expressed in the eyes (black arrowheads in the control, N), revealing the ectopic eyes around the treated animal (M). Anterior is shown to the left in those cases where an anteroposterior axis is present. Yellow/black asterisks indicate the pharynx. d, dorsal view; v, ventral view. Scale bar in A: 270 µm in A,E; 250 µm in C,G,I,K,M,O; 300 µm in all other images.