Fig. 3. Smed-βcatenin1-silenced intact planarians transform to radial-like hypercephalized planarians. (A-C) Stereomicroscope views showing two different stages. (D-S) Several markers were used at different days after Smed-βcatenin1 silencing to characterize the phenotype. Anti-arrestin (D-F), cintillo (G-I), H.10.2f (J-L), synapsin (M-O), Smed-TCEN49 (P,Q) and Smed-HoxD (R,S). (T) Quantification of the different phenotypes observed after Smed-bcatenin1 silencing, showing that the anteriorization started in most of the animals in the posterior region. Anterior is to the left in those cases where an anteroposterior axis is present. Confocal z-projections (D-F,M-O). Yellow asterisks indicate the pharynx. Yellow/black arrows and red arrows indicate the differentiation of anterior structures in normal and ectopic positions, respectively. d, days after silencing. Scale bar in A: 200 µm in B,E,H,K,N,P,R; 300 µm in all other images.