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Figure 4


Fig. 4. lin-23(ot1) mutants display a mild cdc-25.1-dependent intestinal-specific hyperplasia during embryogenesis. (A) DIC/GFP overlays of wild-type and lin-23(ot1) L1 hatchlings expressing the intestinal-specific elt-2::gfp marker. The wild-type larvae hatch with 20 intestinal cells, whereas the lin-23(ot1) mutants typically display mild intestinal hyperplasia (here, 28 intestinal cells). (B) Quantification of intestinal cell number in L1 hatchlings at 20°C (n=40). The cdc-25.1(gf) and lin-23(ot1) hyperplasia are not additive, whereas the loss-of-function cdc-25.1(rr36) intragenic mutation significantly suppresses both the cdc-25.1(rr31) and the lin-23(ot1)-dependent cell cycle defects. Similar results were observed after hypomorphic cdc-25.1(RNAi) treatment of cdc-25.1(rr31) (data not shown) and lin-23(ot1).