Fig. 2. Co-expression of Ascl1, Olig2 and Nkx2-2 in differentiating OLPs at perinatal stages. Images show staining of rat spinal cord for Ascl1, Olig2 and Nkx2-2, together with GalC and MBP at E20.5 (A-H) and P0 (I-O). Boxes in E and M indicate the locations of the areas shown in other panels. D shows a triple-positive cell (merged image on the left). Insets and arrowheads indicate double-positive cells. Asterisks in I indicate Olig2⁺/Ascl1^- cells. Broken lines indicate the boundary between the VZ and MZ. Scale bars: in C,G and L, 50 µm for A-C, F,G and I-L; 100 µm in E,H,N,O; 200 µm in M.