Fig. 3. Expression of Ascl1-GFP transgene in PDGFR⁺ OLPs. Images show transverse sections of E16.5 spinal cords of Ascl1::GFP reporter mice. (A-D'') Ascl1-GFP and PDGFR were stained in green and red, respectively, and cell nuclei were stained with DAPI in blue. (E-H'') Co-staining for Ascl1-GFP (green), PDGFR (blue) and endogenous Ascl1 protein (red). Boxes indicate the locations of the areas shown in other panels. Broken lines in B-B'' indicates the VZ. Arrowheads indicate the co-expression of GFP and PDGFR, whereas arrows indicate GFP^-/PDGFR⁺ (C-C'') or Ascl1^-/GFP⁺/PDGFR⁺ cells (F-F'',H-H''). Scale bars: 50 µm in A-A''; in B'',C'',D'',E,G,F'' and H'', 20 µm for B-B'',C-C'',D-D'',E,F-F'' and H-H''.