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Figure 4


Fig. 4. PLK-1 asymmetry is controlled by A-P polarity cues. (A,B) GFP-PAR-2 embryos stained for PLK-1 (shown alone in the left panels and in red in the merged panels), GFP (shown alone in the middle panels and in green in the merged panels) and DNA (blue). The posterior domain of GFP-PAR-2 is established before PLK-1 distribution becomes asymmetric. (C-J) Embryos of the indicated genotypes stained for PLK-1 (shown alone in the left panels and in red in the merged panels), {alpha}-tubulin (green) and DNA (blue). PLK-1 asymmetry is dependent on the PAR proteins (D-H), but not on GOA-1/GPA-16 (collectively referred to as G{alpha}) or PIE-1 (I,J).