Fig. 6. kan1 suppresses the as1 pgy1 leaf phenotype in Arabidopsis. (A-C) Rosettes of as1 (A), as1 kan1 (B) and as1 kan1 kan2/+ (C). The rosette leaves of as1 kan1 (B) and as1 kan1 kan2/+ (C) have a surface slightly less rippled than as1 (A). (D-F) Rosettes of as1 pgy1 (D), as1 pgy1 kan1 (E) and as1 pgy1 kan1 kan2/+ (F). The as1 pgy1 phenotype is suppressed by kan1, and to a greater extent in a kan1 kan2/+ background, such that as1 pgy1 kan1 kan2/+ rosette leaves are more similar to as1. (G-J) Plants of kan1 kan2 (G), as1 kan1 kan2 (H), pgy1 kan1 kan2 (I) and as1 pgy1 kan1 kan2 (J). The kan1 kan2 phenotype is not suppressed by as1, pgy1, or by as1 pgy1. (K) Quantitative RT-PCR was used to assay expression levels of PHB, PHV, REV, KAN1 and KAN2 in the following genotypes: wild type, pgy1, as1 and as1 pgy1. Results were normalised to ACTIN2, with the value from wild-type plants arbitrarily set to 1.0. Bars indicate the standard deviation between three biological replicates. ANOVA statistical analysis indicates a significant difference between Ler and as1 pgy1 for KAN1 and KAN2 expression. Scale bars: 1 cm in G-J.