Fig. 1. Gene structure and amino acid sequences of Arabidopsis CPC homologs. (A) Sequence alignment of CPC-homologous MYB proteins (CPL3, CPC, TRY, ETC1, ETC2, Os01g43180 and Os01g43230). Red outlined letters indicate identical residues. (B) Phylogenetic tree based on the amino acid sequences. Numbers above branches are genetic distances based on 10,000 bootstrap replicates. The tree was obtained by the neighbor-joining method using Genetyx ver. 11.2.7 software (Genetyx, Tokyo, Japan). (C) Structure of CPC-homologous genes and positions of mutations. The locations of start and stop codons are indicated. Three exon (boxes) and two intron (lines) positions were determined by comparing the genomic sequences with the cDNA sequences. Positions of T-DNA insertions and the identity of mutations are indicated (cpc-2, try-029760, etc1-1, etc2-2 and cpl3-1). (D) Semi-quantitative RT-PCR analysis of CPL3. EF (At1g07930) was used as a control.