Fig. 2. Analysis of STATc activation by DIF-1 and osmotic stress in a PTP3 overexpressing strain. Parental Ax2 cells and PTP3OE cells were starved for 4 hours and then left untreated, exposed to 100 nM DIF-1 (A) for 5 minutes or exposed to 200 mM sorbitol (B) for 5 minutes. One aliquot of cells was lysed, subjected to western transfer and the blot was analysed with an antibody specific to the tyrosine phosphorylated form of STATc. As a loading control, a parallel blot was probed with total STATc antibody. The second aliquot of cells was fixed and immunostained for STATc.