Fig. 6. Electrophoretic mobility shifts of myc-PTP3CS after DIF-1 treatment. Cells transformed with myc:PTP3CS were starved for 4 hours, exposed to DIF-1 at 100 nM for 3 minutes or left untreated, where indicated the samples were treated with lambda phosphatase. All samples were analysed by 2D gel electrophoresis (pH 6-10 for first dimension and 4-12% gradient gel for second dimension). After western transfer, the resultant blots were stained using a myc antibody.