Fig. 4. FRAP analyses of -catenin-GFP. (A) Dorsal branch of btl>-catenin-GFP embryos with or without Src42A^ACT were photo bleached at the bracketed regions and fluorescence recovery was recorded. (B) Schematic plot of FRAP analysis. Photo bleaching reduces the fluorescence level from pre bleach (F_pre) to post bleach (F_post). Fast recovery of fluorescence reaches an approximate plateau level (F_end) that is usually lower than F_pre. Recovery to F_pre required a longer time and was not analyzed. Mobile fraction [M_f=100x(F_end-F_post)/(F_pre-F_post)] and half-time of recovery (t) were estimated from the plots. (C) Representative plots of fluorescence recovery in dorsal branches of control (black), Src42A; Src64B double mutant (red) and Src42A^ACT-expressing (blue) embryos. (D) t and M_f values under various conditions of Src activities. Error bar indicates standard error. Values that deviate from controls are indicated (^*P<0.05, ^**P<0.005).