Fig. 1. p63 and Dlx proteins in the embryonic limbs. (A-D) Whole-mount in situ hybridization on wild-type FLs (A,B) and HLs (C,D) of E10 embryos for Dlx5 (A,C) and Dlx6 (B,D). (D') Hybridization for Dlx6 on wild-type HLs of E11 embryos. Lateral views are shown, the signal is indicated with black arrows. Anterior is to the top. (E-H) Double immunostaining for pan-Dlx (red) and p63 (green) on sections of E10.5 HLs. The merged image is shown in G. (H) Nuclei were counterstained with DAPI. Dashed line indicates the border between the AER and the limb mesenchyme. Section plane and orientation is indicated (I). (J,K) Double immunostaining for pan-Dlx and p63 on sections of the frontonasal region of E11 embryos, counterstained with DAPI. The olfactory placode is shown, dashed boxes indicate the neural and non-neural regions. Colocalization of p63 and Dlx proteins occurs in the non-neural epithelium. Section plane is indicated (L). Dor, dorsal; Ven, ventral; Di, distal; Pr, proximal; OP, olfactory placode. Scale bar: 25 µm.