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Figure 2


Fig. 2. Expression of p63 and Dlx genes in embryonic limbs. (A) Expression of TAp63 and {Delta}Np63 (left), and of Dlx1, Dlx2, Dlx5 and Dlx6 (right) in FLs (top) and HLs (bottom), collected at E10.5 (white bars), E11.5 (grey bars) and E12.5 (black bars). Data are normalized against GAPDH. The E10.5 abundance is set at 1. (B) RT-PCR semiquantitative analysis of each TAp63 ({alpha}, β, {gamma}; left) and {Delta}Np63 ({alpha}, β, {gamma}; right) isoform mRNAs in FLs and HLs at the indicated embryonic ages. For each mRNA, 29 or 38 amplification cycles are shown (indicated on the right). GAPDH mRNA is used as a reference. As a positive control (C+), the cloned cDNA of each isoform was used (right-most lanes). (C) Western blot analysis on total protein extracts from HaCaT cells (expressing {Delta}Np63{alpha}) and FLs collected at E10.5 and E12.5, showing the {Delta}Np63{alpha} protein (black arrowhead). Expression sharply increases between E10.5 and E12.5. β-actin is used for control.