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Figure 5


Fig. 5. Expression of p63 and Dactylyn in Dlx5;Dlx6 DKO limbs. (A,B) Morphology of E11 wild-type (A) and Dlx5;Dlx6 DKO (B) HLs. Section plane is shown in A. (C-F) Immunostaining for p63 on sections of normal (C,E) and Dlx5;Dlx6 DKO (D,F) FLs (top panels) and HLs (bottom panels) at E11. Nuclei were counterstained with DAPI. The AER is indicated with white arrows. The dorsoventral orientation is indicated. (G) Dissection of the medial (Me) sector from wild-type and Dlx5;Dlx6 DKO limbs. (H) TAp63, {Delta}Np63 and Dactylyn mRNA abundance in the Me sector of wild-type (white bars) and Dlx5;Dlx6 DKO (black bars) FLs and HLs, by RealTime qPCR. Wild-type samples are set=1. Scale bar: 25 µm.