Fig. 1. Sdmg1 is a transmembrane protein expressed in male embryonic gonads. (A) RT-PCR showing male-specific expression of Sdmg1 in 13.5 dpc gonads. RT-PCR for Gapdh and RT-PCR without reverse transcriptase (RT) are shown as controls. (B) Transmembrane helix prediction for Sdmg1. The DUF300 domain is indicated with a black bar. (C) Topology of Sdmg1. NIH3T3 cells were transiently transfected with HA-Sdmg1-YFP (green) and immunostained with anti-HA or anti-YFP antibodies (red). Antibody accessibility indicates that the N-terminal HA epitope is extracellular and the C-terminal YFP epitope is intracellular. Scale bar: 10 µm.