Fig. 5. Sdmg1 is required for membrane trafficking and secretion in SK11 Sertoli cells. (A-F) Immunostaining of undifferentiated SK11 Sertoli cell lines shows that lines stably transfected with Sdmg1 knock-down constructs (Sdmg1 shRNA and Sdmg1 miRNA) have reduced levels of Sdmg1 protein. (G-L) Immunostaining of SK11 Sertoli cells after 2 days of differentiation shows that Stx2 accumulates intracellularly in lines stably transfected with Sdmg1 knock-down constructs. DNA is shown in red. Scale bars: 50 µm. (M-O) Histology of 11.5 dpc female urogenital ridge tissue cultured after aggregation with or without SK11 Sertoli cell lines. Most germ cells develop into female meiotic oocytes after aggregation without SK11 cells (M, arrowheads), into male prospermatogonia (N, arrow) after aggregation with SK11 cells, and into female meiotic oocytes after aggregation with Sdmg1 knock-down SK11 cells (O, arrowheads). Scale bar: 10 µm.