Fig. 5. Ush1 protein localization in early developing Ush1 mutant hair bundles. (A) Hair bundles of sensory cells from the cochlear basal turn of E18.5 Myo7a^{4626SB/4626SB}, Cdh23^v2J/v2J, Pcdh15^av3J/av3J and Ush1g^js/js mice stained with the harmonin-H1b antibody (green) and phalloidin (red). Note the absence of signal in the hair bundles of Ush1g^js/js and Myo7a^{4626SB/4626SB} mice, and the presence of immunoreactive spots in the cuticular plate of the latter (arrowheads). (B) Hair bundles of sensory cells from the cochlear basal turn of E17.5 wild-type mice and Ush1c^-/- mice that lack harmonin. Whole-mount cochleas were labeled with phalloidin (red) and antibodies to myosin VIIa (Myo7a-F1), harmonin-b (harmonin-H1b), cadherin 23a (Cdh23-N1) or protocadherin 15a/b (Pcdh15-cter) (green). Cdh23-a and Pcdh15-a/b are detected along the stereocilia shafts both in wild-type and Ush1c^-/- hair bundles. Note the absence of a Myo7a-F1 signal at stereocilia tips in Ush1c^-/- hair cells (arrowheads). Scale bars: 2 µm.