Fig. 7. Mouse Ush1 protein localization in late developing Ush1 mutant hair bundles. (A) Hair bundles of sensory cells from the cochlear basal turn of P5 Myo7a^{4626SB/4626SB}, Cdh23^v2J/v2J, Pcdh15^av3J/av3J and Ush1g^js/js mice were stained with the harmonin-H1b antibody (green) and phalloidin (red). Note again the absence of harmonin-b labeling in hair bundles of Ush1g^js/js and Myo7a^{4626SB/4626SB} mice, and the presence of immunoreactive spots that have increased in number and size since E18.5, in the cuticular plate of the latter mutant (arrowheads). In Cdh23^v2J/v2J and Pcdh15^av3J/av3J mice, harmonin-b is also mislocated, as it is still detected at stereocilia tips (arrowheads) instead of near the tip link side attachment point (see wild-type control in B). (B) Cochleas of P5 wild-type and Ush1c^-/- mice were stained with phalloidin (red) and antibodies to myosin VIIa (Myo7a-F1), harmonin-b (harmonin-H1b), cadherin 23a (Cdh23-N1) or protocadherin 15a/b (Pcdh15-cter) (green). The stainings for myosin VIIa, Cdh23-a and Pcdh15-a/b are the same in Ush1c^-/- and wild-type hair bundles. Scale bars: 2 µm.