Fig. 2. APC/C^Fzr/Cdh1 functions during endocycles. (A) Schematic representation of Flipout/Gal4 clones induction in the Drosophila salivary gland. Clones are induced during the first larval instar, after the cells have entered the endocycle (Smith and Orr-Weaver, 1991). The Flipout/Gal4 technique was used to clonally express UAS-Apc^RNAi constructs with GFP. (B-D''') salivary gland containing (B-B''' and C-C''') Apc8^RNAi or (D-D''') Apc8^RNAi; Apc10^RNAi Flipout/Gal4 clones stained with (B,C,D, white; B''',C''',D''', green) -GFP, (B',D', white; B''', D''', red) -Geminin and (C', white; C''', red) -Cyclin A antibodies and (B'',C'',D'', white; B''',C''',D''', blue) DAPI. (B',D') Geminin and (C') Cyclin A are ectopically expressed in cells that express the Apc8^RNAi or the Apc8^RNAi; Apc10^RNAi constructs. Expression of Apc8^RNAi, as well as the co-expression of Apc8^RNAi and Apc10^RNAi, results in a decrease in the nuclear size (B'', arrowheads; D'', arrows).