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Figure 3


Fig. 3. Cyclin E overexpression during the endocycle results in the unscheduled accumulation of APC/C targets. The Flipout/Gal4 technique was used to clonally overexpress Cyclin E and Fizzy-related with GFP. (A-B''') Drosophila salivary glands and (C-D'') egg chambers containing (A-C'') UAS-Cyclin E and (D-D'') UAS-Cyclin E; UAS-Fzr Flipout clones labeled with (A,B, white; A''',B''',C,C'',D,D'', green) {alpha}-GFP, (A', white; A''',C',C'',D',D'', red) {alpha}-Geminin and (B', white; B''', red) {alpha}-Cyclin A antibodies and (A'',B'', white; A''',B''',C-C'',D-D'', blue) DAPI. (A',C') Geminin and (B') Cyclin A are ectopically expressed in cells that overexpress Cyclin E, both in the salivary gland (arrows) and in follicle cells of the ovary (brackets). (D-D'') In follicle cells, the co-expression of Fzr/Cdh1 with Cyclin E inhibits the expression of Geminin (brackets).