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Figure 4


Fig. 4. Expression of the Fzr/Cdh1 inhibitor Rca1 during the endocycle. Rca1 was ectopically expressed using either (A-C) the Flipout/Gal4 technique or (D) a hs-Rca1 construct. (A-B''') Drosophila salivary glands and (C-C'') egg chamber containing UAS-Rca1 Flipout clones stained with (A,B, white; A''',B''',C,C''', green) {alpha}-GFP, (A', white; A''',C',C'', red) {alpha}-Geminin and (B', white; B''', red) {alpha}-Cyclin A antibodies and (A'',B'', white; A''', B''',C,C',C'', blue) DAPI. (D-D'') Egg chambers expressing a hs-Rca1 construct labeled with (D, white; D'', red) {alpha}-Cyclin A and (D', white; D'', green) {alpha}-Geminin antibodies and (D'', blue) DAPI. Induction of Rca1 expression leads to the accumulation of APC/C targets, in the (A,B) salivary gland (arrows), in the (C) follicle cells (brackets) and in (D) the nurse cells.