Fig. 5. Co-expressing a Geminin^RNAi construct does not rescue the Cyclin E or Apc^RNAi overexpression phenotypes. The Flipout/Gal4 technique was used to clonally overexpress Geminin, Cyclin E Apc10^RNAi; Apc8^RNAi and Geminin^RNAi with GFP. Drosophila salivary glands containing (A-A''') UAS-Geminin, (B-B''') UAS-Cyclin E; UAS-Geminin^RNAi and (C-C''') UAS-Apc10^RNAi/UAS-Geminin^RNAi; UAS-Apc8^RNAi Flip-out clones labeled with (A,B, white; A''',B''', green) -GFP, (C, white; C''' green) -β-Gal and (A',B',C', white; A''',B''',C''', red) -Geminin antibodies, and (A'',B'',C'', white; A''', B''',C''' blue) DAPI. (A-A''') The overexpression of Geminin, in cells marked by an asterisk, blocks DNA replication. (B-B''',C-C''') Co-expression of Geminin^RNAi does not rescue the block of DNA replication associated with overexpression of Cyclin E or Apc10^RNAi; Apc8^RNAi [compare the DNA content in flip-out clones (arrows) with the DNA content in wild-type cells (arrowheads)]. The mitotic cells of the imaginal ring cyclically express Geminin (asterisk).